Effect of Mycoplasma synoviae and lentogenic Newcastle disease virus coinfection on cytokine and chemokine gene expression in chicken embryos
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چکیده
منابع مشابه
The Tween 80 Toxicity in Chicken Embryos and Effect on the Kinetics of Newcastle Disease Virus Replication
Background: Non-ionic surfactant, Tween-80 (TW80) is commonly used for drug delivery due to its effect on the cell membrane permeation. The change in permeability can also increase viral infectivity in cells. This study was undertaken to improve upon Newcastle disease virus (NDV) titer cultivated with embryonic chicken eggs. Methods: The toxicity of TW80 was investigated against chicken embryo...
متن کاملMycoplasma synoviae infection on Newcastle disease vaccination of chickens
Newcastle disease is characterized by respiratory manifestations in association with nervous and/or digestive symptoms. Its prevention is done by vaccination with live attenuated (lentogenic strains) and/or killed vaccines. The lentogenic strains can lead to strong post-vaccination reaction, principally due to the presence of other pathogenic agents. Among them, Mycoplasma synoviae is worldwide...
متن کاملInduction of Intestinal Immune Response to Lentogenic Vaccine Strain of Newcastle Disease Virus in Chicken
Mucosal surface provide portal entry for a majority of infectious agents. Hence, protection at the mucosal surface through immunization plays an important role in containing its systemic spread. Mucosal vaccination of chicken at early stages of life with live attenuated strain of Newcastle disease virus (NDV) induces both cellular and humoral immune responses against the Newcastle disease. In o...
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تعداد 20 رأس میش نژاد نائینی 6+-24 ماهه، با میانگین وزن 2/3-+40 کیلوگرم، همراه با 20 رأس بره های آنها در قالب طرح کاملا تصادفی اثر بتائین و مکمل سولفات بر خصوصیات شیر و پشم و عملکرد بره ها بررسی شد. میش ها بطور تصادفی در چهار تیمار قرار گرفتند. تیمارها عبارت بودند از: 1-گروه شاهد 2-بتائین (05/0 درصد ماده خشک) 3-سولفات (24/0 سولفور درصد ماده خشک 4-بتائین هرماه با سولفات. جیره غذائی طبق nrc با مح...
15 صفحه اولDetection of Newcastle Disease Virus in Infected Chicken Embryos and Chicken Tissues by Rt-pcr
RT-PCR for the detection of Newcastle disease virus (NDV) in allantoic fluids of SPF embryonated eggs as well as in tissues of SPF chickens infected experimentally is described. The method proved to be specific as all tested NDVs were detected and no cross reaction with other RNA viruses was observed. Sensitivity of the method was established at 10ELD50/0.1 ml. To detect NDV in chicken tissues,...
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ژورنال
عنوان ژورنال: Poultry Science
سال: 2013
ISSN: 0032-5791
DOI: 10.3382/ps.2013-03332